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ARCaP EMT model in which selected growth factors or bone microenvironment drives ARCaPE cells to undergo mesenchymal-like transition to ARCaPM. Upon this morphologic transition (a), ARCaPM cells expressed both classic EMT markers (decreased expression of E-cadherin and cytokeratins 18/19 and increased expression of vimentin and N-cadherin) and novel markers (increased expression of RANKL and IL13Rα2) as analyzed by RT-PCR (b), western blot (c) and IHC (d)
