Fig. 5.
Combination treatment of ARCaPE.cells with TGFβ1 and EGF induced the protein expression of Snail and RANKL (left panels). Cells were treated with EGF (50 ng/ml) plus TGFβ1 (4 ng/ml) for 3 days (E + T). Note that ARCaPM cells expressed higher levels of endogenous Snail and RANKL than ARCaPE (lanes denoted with C). Right panels: over-expression of Snail induced RANKL expression in the ARCaPE cells. An expression vector containing a constitutively active Snail (Flag-Snail-6SA) was used. EV1–3: vector controls; clones 1–8: stable Snail transfected clones