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. Author manuscript; available in PMC: 2011 Mar 8.
Published in final edited form as: Clin Exp Metastasis. 2008 Jun 6;25(6):601–610. doi: 10.1007/s10585-008-9183-1

Fig. 6.

Fig. 6

LIV-1 plays an important role in ARCaP EMT. (a) LIV-1 antibody recognized a single band at 85 kDa and was expressed at higher levels in ARCaPM than in ARCaPE cells and is sensitive to siRNA targeting LIV-1 at both RNA and protein levels. Since this was a transient knockdown study, we observed only a slight change of LIV-1 protein level possibly due to the longer t1/2 of this protein in comparison to LIV-1 mRNA. (b) LIV-1 transfected ARCaPE cell clones expressed increased Snail and decreased E-cad, an indication of mesenchymal-like transition. (c) LIV-1 siRNA treated ARCaPM cells expressed decreased levels of LIV-1, Snail, N-cad, vimentin and RANKL, but increased E-cad, indicative of epithelial differentiation in comparison to untreated or scrambled LIV-1 siRNA-treated ARCaPM cells