Figure 2.

Spatiotemporal localization of the host response to grafted injuries in transgenic reporter mice. Transgenic mice expressing the firefly luciferase reporter under the regulation of either (A) the vascular endothelial growth factor receptor 2 promoter or (B) the Smad binding element promote were analyzed for the kinetics of the vascular remodeling and scarring response, respectively, 3, 7 and 14 days after placement of a full-thickness Integra^® graft. Transgenic expression of luciferase was imaged and bioluminescence quantified by selecting a circular region of interest (ROI) at the grafting site and comparing it to an ROI at a site remote form the injury.