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. 2011 Feb 17;4:7. doi: 10.1186/1755-1536-4-7

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Copyright ©2011 Sawada et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phylogenic tree of peptide targeting candidates eluted after non-fat milk (NFM) or no blocking of matrix sonicated samples. Peptide sequences were obtained after the third round of screening using the IT9 (PHD 12) protocol, with NFM used to block the non-specific binding to sonicated matrix. All 30 clones analyzed in the NFM-free assay corresponded to a single peptide of 12 amino acids: AHKHKPGHITA. Structural relatedness using MacVector sequence analysis software. Recovery of increased 800-fold in IT9 biopanning.