Table 3.
Identification of Integra® (IT) matrix targeting peptides from peptide libraries after bovine serum albumin (BSA), phosphate-buffered saline (PBS) and non-fat milk (NFM) blockinga
| IT8b | R3 (pH 2) | IT8 | R3 (NaCl) | IT8 | R3 (pH 2) | IT8 | R3 (NaCl) | IT8 | R4 (pH 2) | IT8 | R4 (pH 2) | IT9c | R4 (pH 2) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BSA | IT8-BA | BSA | IT8-BAS | PO4 | IT8-PA | PO4 | IT8-PAS- | BSA | IT8-BA | PO4 | IT8-PA- | NFM | IT9-MA |
| Figure 6A | Figure 6B | Figure 6C | Figure 6D | Figure 7 | |||||||||
| BA1 | CVPSSARIC | BAS1 | CHMSPRHQS | PA1 | CVQSSTQHC | PAS4 | CHVTAQRAC | R4BA1 | CKGPVSRHC | R4PA1 | CGKHDDTYC | MA1 | HETFPSPRANSV |
| BA2 | CRPHDSKAC | BAS2 | CLPNKQWSC | PA2 | CSGHHSLRC | PAS5 | CATPEWPPC | R4BA2 | CTTSSEHVC | R4PA2 | CPTKDLRYC | MA2 | HETFPSPRANSV |
| BA3 | CHPEPRSQC | BAS3 | CKQPLNNTC | PA3 | CPTSQQKVC | PAS6 | CPNLMNTRC | R4BA3 | CSTTMKTSC | R4PA3 | CTSSGNRYC | MA3 | HETFPSPRANSV |
| BA4 | CVEKRPRQC | BAS4 | CTVTPRHLC | PA4 | CNSTHPRAC | PAS8 | CTKSSPPRC | R4BA4 | CDNKRSPAC | R4PA4 | CTIKTNLQC | MA4 | HETFPSPRANSV |
| BA5 | CFMDYRNLC | BAS5 | CDNTSKTQC | PA5 | CNRLESHLC | PAS9 | CTQTTVASC | R4BA5 | CKLNYPNAC | R4PA5 | CHSTAKSAC | MA5 | SQIDYATGPRQA |
| BA6 | CSHSVQPFC | BAS6 | CASTTAACC | PA6 | CTNPHRSQC | PAS10 | CDQSKTIAC | R4BA7 | CQFSKSQSC | R4PA6 | CPASKGDFC | MA6 | WDTEKASPLSPL |
| BA7 | CQTHNPRQC | BAS7 | CLHMDKKRC | PA7 | CTKTPWPGC | PAS11 | CSRGSMGIC | R4BA8 | CTLDTRRDC | R4PA8 | CSHRVPHDC | MA7 | HETFPSPRANSV |
| BA8 | CDGAPAPLC | BAS8 | CMKTPMRSC | PA8 | CNRLQGEHC | PAS12 | CSPIRGSMC | R4BA10 | CPFSSSPSC | R4AP9 | CHATPYPKC | MA8 | HETFPSPRANSV |
| BA10 | CKTDLQKQC | BAS10 | CYKHVGQRC | PA9 | CPTPTGRYC | PAS13 | CSHTGHHQC | R4BA11 | CPSMSHHQC | R4PA10 | CDSSRHTHC | MA10 | DHTGKSPGLFHN |
| BA11 | CGPFPQPHC | BAS11 | CHLSPFKSC | PA10 | CVPTAMSNC | PAS14 | CHEPTTMAC | R4BA12 | CSASTQSFC | R4PA11 | CSRLSQEYC | - | - |
| BA12 | CSFHGPGPC | BAS12 | CTTSKYRDC | PA11 | CSLARPNEC | PAS15 | CSRADLTTC | R4BA13 | CPLKGLATC | R4PA12 | CTGKQYPQC | - | - |
| BA13 | CSTNQTPTC | BAS13 | CTATGLSNC | PA12 | CVRTPFSMC | - | - | R4BA14 | CTGKPLKTC | R4PA13 | CGMNAFRAC | A1 | AHKHKHPGHITA |
| BA14 | CSTSPONSC | BAS14 | CPSSMPSRC | PA13 | CNNTTPPSC | - | - | R4BA17 | CIHMTGYHC | R4PA14 | CGMNAFRAC | - | - |
| BA15 | CKLIHNNSC | BAS15 | CPATSHTHC | PA15 | CTSQQKANC | - | - | R4BA21 | CEMTETKHC | R4PA16 | CTTKYSTTC | - | - |
| - | - | - | - | - | - | - | - | R4BA26 | CKENWPLIC | R4PA18 | CSSDKALVC | - | - |
| - | - | - | - | - | - | - | - | R4BA30 | CSNSPTTMC | R4PA24 | CSPRSHLSC | - | - |
| - | - | - | - | - | - | - | - | - | - | R4PA25 | CPPSPMPYC | - | - |
aThe sequences of peptides pulled from the biopanning strategies IT8 and IT9 are presented along with their code numbers for reference.
bIT8 was screened against matrix sonicated samples blocked with BSA or PBS-T, and the peptide sequences were generated from both acid and GAG elutions.
cIT9 was biopanned from thin sections of matrix blocked with NFM or PBS-T, and only the acid/high salt eluates evaluated.