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. 2011 Mar 8;6(3):e17179. doi: 10.1371/journal.pone.0017179

Figure 4. Altered α-actinin expression modifies cell detachment.

Figure 4

A) α-Actinin siRNA knockdown was quantified by RT-PCR (means + SEM of 4 experiments) and Western blot. B) The hexosaminidase activity assay was used to examine the cell detachment after siRNA-treatment. In response to HAMLET, tumor cell detachment was increased in cells with low α-actinin levels compared to cells transfected with negative control-siRNA or untransfected cells (means ± SEMs of 4 experiments, *** p<0.001). C) HAMLET response of cells overexpressing α-actinin-4-GFP was studied by live cell confocal microscopy. Rounding up in response to HAMLET (21 µM) was delayed compared to cells transfected with the GFP plasmid and cells without the α-actinin-4-GFP plasmid. D) Overexpression of α-actinin-4-GFP in A549 cells delays blebbing and nuclear condensation caused by HAMLET (21 µM, 3 hours, means + SDs of 3 experiments, ** p<0.01).