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. 2011 Mar 8;6(3):e17235. doi: 10.1371/journal.pone.0017235

Figure 4. Replication efficiency of NUMT in ARS containing regions.

Figure 4

Transformation efficiency (upper panels) and plasmid loss rate (middle panels) of sequences containing different combinations of an ARS and a NUMT, full sequences or various portions of them (schematized in the lower panels). NUMT13 (69 bp) overlaps by 39 bp the 5′ portion of ARS1215 (229 bp). NUMT30 (23 bp) is located in the middle of ARS1635 (231). NUMT14 (43 bp) is distant 387 bp from proARS408 (1349 bp); the definition of proARS is according to OriDB, since its activity has not been experimentally confirmed. For each of the three NUMT/ARS combinations, the full sequence and various portions of it (schematized in the lower panels) were analysed. For each construction, vectors pIRT2Δ (white bars) and pRS405 (grey bars) were used. The 17 bp ACS motif of NUMTs and ARS is indicated in red; no confirmed or predicted ACS element is reported for proARS408. A star indicates that the ACS motif has been replaced with a G+C rich region. The chromosomal coordinates of NUMTs and ARS are indicated; when portions of the full sequence are analysed, their coordinates are also indicated.