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. 2011 Feb 21;11:79. doi: 10.1186/1471-2407-11-79

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Copyright ©2011 Valentine et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nutlin-3 leads to phosphorylation of several important DDR mediators. (A) HCT116^p53+/+cells were untreated (treated with DMSO only) (unt) or treated with 100 μM Etoposide (Eto) or 10 μM Nutlin-3 (Nut) for the times indicated before immunoblotting was used to analyse phosphorylation of p53 at Ser15, Ser20 and Ser37. Actin levels were used to assess equal loading. (B) HCT116^p53+/+cells were untreated (treated with DMSO only) (unt) or treated with 10 μM Nutlin-3 (Nut) or 100 μM of Etoposide (Eto) for 1 hour before the phosphorylation of ATM (Ser1981), BRCA1 (Ser1542) and CHK2 (Thr68) were analysed using immunoblotting. Actin levels were used to assess equal loading. (C) HCT116^p53-/-cells were untreated (treated with DMSO only) (unt) or treated with 10 μM Nutlin-3 or 100 μM of Etoposide (Eto) for 1 hour before the phosphorylation of ATM (Ser1981) and BRCA1 (Ser1542) were analysed using immunoblotting. Actin levels were used to assess equal loading.