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. 2011 Feb 11;10:16. doi: 10.1186/1476-4598-10-16

Figure 1.

Figure 1

sFZD7 binds to Wnt3 and suppresses Wnt3-induced β-catenin/Tcf4 transcriptional activation in Huh7 cells. (A). SDS-PAGE analysis of purified sFZD7. (B). Pull-down assay demonstrating the interaction between sFZD7 and Wnt3. Huh7 cells lysate was pulled down with Ni-NTA agarose with or without recombinant His-sFZD7 peptide. Protein complexes were centrifuged and eluted, and the supernatants analyzed by Western blot using rabbit anti-Wnt3 antibody (upper panel) and anti-His antibody (lower panel). Huh7 lysate and recombinant His-sFZD7 were used as positive controls. (C). sFZD7 abolished the Wnt3-induced β-catenin/Tcf4 transcriptional activity in Huh7 cells. Huh7 cells were transfected with 0.2 μg of each of the indicated expression plasmid, along with 0.3 μg of pTOPFLASH or pFOPFLASH reporter plasmids and 0.1 μg of β-gal to normalize for transfection efficiency. The results are expressed as means ± SD (error bars) of triplicate assays. *P < 0.05 versus control.