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Human bronchial and lung epithelial cells express S100A7/psoriasin mRNA. RT-PCR was performed with specific primers for S100A7/psoriasin and PBG-D as an internal control on NCI-H727 and A549 cells. The expected size of PCR products is 248 bp for S100A7/psoriasin and 117 bp for PBG-D. PCR products were separated on 1.5% agarose gel containing ethidium bromide. PCR reaction in absence of cDNA was used as a negative control, plasmid DNA as a positive control, 100-bp ladder as Marker.
