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. 2011 Feb 15;11:10. doi: 10.1186/1471-2466-11-10

Figure 2.

Figure 2

S100A7/psoriasin mRNA levels remain unchanged after treatment with Toll-like receptor ligands or proinflammatory cytokines. NCI-H727 (upper panel) and A549 cells (lower panel) were treated with 100 ng/ml LPS, 50 U/ml IL-1β, 5 ng/ml TNF-α, or medium alone (Control) for the time indicated. CXCL-8 mRNA (left) and S100A7/psoriasin mRNA (right) were analyzed by quantitative real-time PCR, and data are presented as mRNA level of both genes relative to PBG-D. CXCL-8 release (middle) was determined by ELISA. All values are depicted as mean ± SEM of three independent experiments.