Figure 1. Expression of inhibin α-, β_A- and β_B-subunits in the postnatal mouse ovary.

Basal expression levels of inhibin α- (A), β_A- (B) and β_B- (C) subunits in developing mouse ovaries were determined by real-time RT-PCR. The mRNA levels of the subunits are shown as a ratio over the day 2 ovary mRNA levels (control). Detection of the inhibin α- (D), β_A- (E) and β_B- (F) subunit protein in ovarian lysates from day 6, day 10 and day 19 ovaries. Ovarian lysates were collected, separated under reducing conditions; each subunit was detected by immunoblotting with the corresponding rabbit polyclonal antibody. Forty µg of protein was loaded per lane. Equal loading of lysates was confirmed with an anti-actin antibody.