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. 2011 Mar 8;6(3):e17348. doi: 10.1371/journal.pone.0017348

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Antenos et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of the wild type inhibin α-subunit (RARR) or the α-subunit with modified cleavage recognition sites (RARA and RAAA) in HEK 293 cells. Culture media were examined by immunoblot analysis under reducing conditions using an anti-inhibin α-subunit antibody. Processing of the inhibin β_B-subunit (B) and inhibin α-subunit (C) by proconvertases. LoVo cells were transiently transfected with expression plasmids for furin, PC5/6A, PC5/6B, Pace4 and PC7. Media from cells was TCA-precipitated, separated by SDS-PAGE under reducing conditions and examined by immunoblot analysis. The corresponding % of band intensities were deduced from the ratio of (β_B)/(pro-β_B+β_B) for the β_B-subunit; or (αC)/(pro-αN-αC+αC) for the α-subunit. Results are representative of two independent experiments.