Figure 1. Effect of V326N mutation on Als5p expression and aggregation in Saccharomyces cerevisiae.

(A) Maps of Als5p^WT and Als5p^V326N. The open reading frame is 1419 amino acid residues long, and the mutation is near the N-terminus of the 103-residue T domain. (B) Immunofluorescence analysis shows expression of V5-tagged Als5p^WT and Als5p^V326N in S. cerevisiae. (C) Adherence and aggregation of yeast cells (gray) to heat denatured BSA-coated beads (brown-gold) for S. cerevisiae without Als5p (EV) or expressing Als5p^WT or Als5p^V326N. Images shown were visualized using bright field microscopy. The diameter of the beads is 2.8 µm.