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. 2011 Mar 8;5(3):e1009. doi: 10.1371/journal.pntd.0001009

Figure 8. The female reproductive system.

Figure 8

(A) Top, female labeled with DAPI and phalloidin to show various regions of the female reproductive system. Autofluorescence from the vitelline cells is shown in green; this autofluorescence served as a useful marker for the vitelline duct and its contents. Image represents tiled images of confocal sections from a single animal. Yellow and cyan boxes correspond to the regions of the seminal receptacle and the ootype/Mehlis' gland complex, respectively. These boxes are color coded to indicate the relative positions of the structures shown in either panels B (yellow bar in upper left) or C (cyan bar in upper left). (B) Left, anti-acetylated α-tubulin labeling microtubules of sperm present in the seminal receptacle. Right, large nuclei of oocytes in the ovary can be seen stained with DAPI to the left. Dashed line represents the position of the of muscle layer surrounding the ovary. Images are from mid-level maximum intensity projections. (C) Maximum intensity projections showing staining of the ootype (top, shown surrounding an egg) with sWGA and Mehlis' gland with PNA, sWGA and acetylated α-tubulin (bottom). Arrowheads indicate cytoplasmic projections of Mehlis' gland cells. (D) Magnified view of Mehlis' gland from panel C. Scale Bars, 10 µm. Anterior faces left in panel A and up in panels B, C and D.