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. 2011 Mar 8;6(3):e14753. doi: 10.1371/journal.pone.0014753

Table 1. Frequency of observed maternal behaviors in inbred and reciprocal hybrid mothers.

BEHAVIOR C57BL/6 BALB/c F (1,50) P-value B6xC CxB6 F(1,75) P-value
Arched-back nursing 0.312±0.025 0.158±0.021 25.049 0.001 0.363±0.021 0.416±0.020 3.392 n.s.
Blanket nursing 0.270±0.028 0.428±0.028 15.763 0.001 0.206±0.024 0.172±0.018 1.357 n.s.
Licking/grooming pups 0.137±0.007 0.045±0.003 150.853 0.001 0.130±0.006 0.091±0.004 30.306 0.001
Self grooming (in nest) 0.014±0.002 0.019±0.003 1.731 n.s. 0.022±0.002 0.026±0.002 2.498 n.s.
Sniffing nest 0.029±0.003 0.012±0.001 31.594 0.001 0.007±0.001 0.009±0.001 2.672 n.s.
Self grooming (out of nest) 0.029±0.007 0.026±0.003 0.128 n.s. 0.031±0.002 0.034±0.003 0.698 n.s.
Sniffing cage 0.052±0.004 0.067±0.006 3.853 n.s 0.071±0.004 0.073±0.004 0.090 n.s.
Eating 0.079±0.005 0.119±0.007 21.747 0.001 0.085±0.005 0.083±0.004 0.088 n.s.
Carrying pup 0.001±0.000 0.000±0.000 6.683 0.013 0.000±0.000 0.000±0.000 0.947 n.s.
Moving pups 0.002±0.000 0.006±0.001 17.920 0.001 0.001±0.000 0.002±0.000 0.383 n.s.
Nest Bulding 0.012±0.002 0.010±0.001 0.506 n.s. 0.007±0.001 0.006±0.001 0.187 n.s.
Sniffing pups 0.010±0.001 0.012±0.001 0.786 n.s. 0.005±0.001 0.010±0.001 17.214 0.001
Drinking 0.015±0.002 0.021±0.002 4.021 n.s. 0.022±0.001 0.020±0.002 0.832 n.s.
Rearing 0.006±0.001 0.012±0.001 9.479 0.003 0.006±0.001 0.007±0.001 1.193 n.s.
Digging 0.016±0.002 0.039±0.005 15.640 0.001 0.014±0.002 0.015±0.002 0.154 n.s.
Carrying tail 0.006±0.001 0.008±0.002 0.778 n.s. 0.002±0.000 0.003±0.001 1.133 n.s.
Climbing 0.008±0.002 0.006±0.002 0.884 n.s. 0.006±0.001 0.003±0.001 3.092 n.s.
Arched-back nursing (<half litter) 0.000±0.000 0.001±0.000 1.207 n.s. 0.001±0.000 0.000±0.000 2.243 n.s.
Licking/grooming pups (<half litter) 0.000±0.000 0.001±0.000 1.859 n.s. 0.000±0.000 0.000±0.000 0.057 n.s.
Blanket nursing (<half litter) 0.000±0.000 0.004±0.004 1.337 n.s. 0.001±0.000 0.001±0.001 0.095 n.s.
Sleeping 0.000 ± 0.000 0.011 ± 0.038 7.989 0.007 0.017±0.003 0.026±0.004 2.683 n.s.

Mean frequencies (± SEM) of an ethogram of twenty-one observed maternal behaviors for C57BL/6 vs. BALB/c (left) and B6xC vs. CxB6 (right) mothers. Behaviors were scored once each minute for two non-contiguous hours each day from postnatal day 1 to 7. Statistical significance was calculated by univariate ANOVA and significant differences (P<0.05) are indicated in bold.