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. 2011 Mar 8;5(3):e976. doi: 10.1371/journal.pntd.0000976

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Pissetti et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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TNF-alpha was assayed by ELISA in supernatants of PBMC cultured for 48 h in the absence of any stimulus and in the presence of T. cruzi antigen, LPS and PHA. Subjects were grouped according to TNF-238A genotypes (TNF-238AA, TNF-238GA and TNF-238GG). Individuals with positive and negative serology were analyzed. Results are expressed as pg/mL. The horizontal line indicates the median, bars the 25% and 75% percentiles, and vertical lines the 10% and 90% percentiles.