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. 2011 Mar 8;5(3):e976. doi: 10.1371/journal.pntd.0000976

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Pissetti et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Percentage of TNF-alpha-producing cells among the CD8⁺ and CD4⁺ population of patients with the cardiac (gray bars) and indeterminate form (white bars) after 48 h of culture in the presence of T. cruzi antigens. B. Percentage of TNF-alpha-producing cells among the CD14⁺ population of patients with the cardiac (gray bars) and indeterminate form (white bars) after 48 h of culture in the presence of T. cruzi antigens. For analysis, a gate was established using FSC and surface marker flowed by analysis of intracellular TNF-alpha expression.