Figure 2.
Decay of PN (blue trace) and formation of nitrotyrosine (black trace) in the reaction of 10 μM MnSOD with 13 μM of PN in the presence of 0.1 mM CO2 in 0.1 M potassium phosphate buffer at pH 7.4 and 25°C. Red trace shows the time course of the nitrotyrosine formation without CO2. In addition, formation of nitrotyrosine in the reaction of 10 μM MnSOD with 18 μM PN, in the absence of CO2, in 0.1 M potassium phosphate buffer at pH 7.4 and 25°C is shown (during the first 2 s).