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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Free Radic Biol Med. 2011 Jan 21;50(8):1000–1008. doi: 10.1016/j.freeradbiomed.2011.01.018

Figure 3. Effect of oxidants on microtubule polymerization.

Figure 3

Tubulin samples (25 μM, 500 μM cys, final reaction volume = 50 μl) were treated with each oxidant for 10 min at RT and excess oxidant was scavenged with 1 mM met. GTP (1 mM final) was added and the samples were incubated at 37 °C for 22 min. Microtubule polymer was collected by centrifugation at 16000 x g for 20 min. The protein concentration in each supernatant was measured using the BCA reagent. The results represent the mean of at least three independent experiments performed in duplicate.

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