Table 1.
Comparison of predicted proteins of the ptl, pen, and pnt biosynthetic gene clusters.
| Ptl (aa)a | Pen (aa, % identity/similarity to Ptl) | Pnt (aa, % identity/similarity to Ptl)) | Pen:Pnt (% identity/similarity | Known or predicted function |
|---|---|---|---|---|
| Sav_2989 (161) | PenR (153, 75/88) | PntR (151, 73/85) | 82/89 | predicted MarR family transcriptional regulator |
| Gap1 (334) | GapN (336, 63/76) | GapR (334, 88/92) | 60/75 | glyceraldehyde-3-phosphate dehydrogenase |
| PenM (398) | PntM (398) | 81/87 | predicted cytochrome P450 monooxygenase | |
| PtlH (285) | PenH (283, 73/83) | PntH (283, 73/84) | 89/94 | 1-deoxypentalenic acid 11β-hydroxylase |
| PtlG (484) | PenG (484, 65/76) | PntG (484, 65/77) | 84/90 | predicted transmembrane efflux protein |
| PtlF (270) | PenF (275, 69/80) | PntF (282, 70/80) | 91/94 | 1-deoxy-11β-hydroxy-pentalenic acid dehydrogenase |
| PtlE (594) | PenE (584, 70/81) | PntE (589, 69/82) | 93/96 | Baeyer-Villiger monooxygenase |
| PtlD (306) | PenD (298, 65/82) | PntD (299, 64/81) | 86/93 | predicted dioxygenase |
| PtlC (58) | hypothetical protein | |||
| PtlB (337) | PenB (337, 78/84) | PntB (337, 76/83) | 93/94 | predicted polyprenyl diphosphate synthase |
| PtlA (336) | PenA (337, 76/86) | PntA (337, 76/87) | 91/95 | pentalenene synthase |
| PtlI (449) | PenI (463, 74/87) | PntI (457, 74/86) | 89/95 | cytochrome P450 monooxygenase |
| PtlR (153) | putative AraC-family transcriptional regulator | |||
| PtlJ (144) | putative lyase | |||
| PtlL (256) | hypothetical protein |
a
For annotations of the ptl cluster, see cited papers as well as ref. (60,61) and the S. avermitilis Genome Project website http://avermitilis.ls.kitasato-u.ac.jp/metabolite/.