A. Real-time quantitative RT-PCR was used to assay the expression of miR-483-3p in tumor samples, either benign (n=24) or malignant (primary, n=10). MiR-483-3p expression, expressed as the ΔCt (Ct miR-483-3p – Ct RNU48), is plotted against the ΔCt of miR-483-5p for each patient sample. A strong positive correlation was detected (r=0.965, Pearson correlation). B. Real-time quantitative RT-PCR was used to assay the expression of IGF2 mRNA in tumor samples, either benign (n=19) or malignant (primary, n=2; ACC metastasis, n=27; ACC recurrence, n=3). IGF2 expression, as the ΔCt (Ct
IGF2 – Ct GAPDH), is plotted against the ΔCt of miR-483-5p (Ct miR-483-5p – Ct RNU48) for each patient sample. A significant positive correlation was detected (r=0.799, Pearson correlation).