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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Jan 26;50(10):1607–1617. doi: 10.1021/bi1013744

Figure 3.

Figure 3

Aβ1–40 kinetic aggregation assay utilizing wild type C. elegans worm PDS with known quantities of preformed Aβ1–40 amyloid fibrils added as seeds. (a) Amyloidogenesis t50 of an Aβ1–40 kinetic aggregation assay without or with the addition of known quantities of Aβ fibrils added to worm PDS. The assay was performed as described in Figure 2. Triplicate data are reported as mean ± SD. A P value of < 0.004 is observed when comparing the mean t50 values in the absence and presence (0.43 ng/mL) of Aβ seeds (indicated with an * in the figure) utilizing the PK and PI treatment protocol (filled triangles). In contrast, a P value > 0.07 between the mean t50 values in the absence and the presence (0.43 ng/mL) of Aβ seeds is observed without PK and PI treatment (unfilled triangles). (b) Dilution series of Aβ1–40 amyloid spiked into worm PDS as detected by Western blot analysis using the 6E10 antibody (antigen retrieval procedures not employed).