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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Jan 26;50(10):1607–1617. doi: 10.1021/bi1013744

Figure 4.

Figure 4

Aβ1–40 kinetic aggregation traces with added mouse brain homogenate. Triplicate results are shown in each figure. (a) Wild type (WT) or AD mouse brain homogenate without PK treatment. Inset: t50 of the kinetic aggregation traces. Data are reported as mean ± SD of triplicate results. (b) WT or AD mouse brain homogenate with PK and PI treatment. Inset: t50 of the kinetic aggregation traces with data reported as mean ± SD of triplicate results. (c) WT, Igf1r+/−, AD and AD;Igf1r+/− mouse brain homogenates with PK and thermal denaturation treatment. The mouse brain homogenates were sonicated for 40 min, treated with PK for 2 h, then boiled for 10 min, sonicated for an additional 20 min, and added to the Aβ1–40 kinetic aggregation assay. Inset: t50 of the kinetic aggregation traces with data reported as mean ± SD. p < 0.013 between AD and AD;Igf1r+/− (analyzed using a two-tailed student's t-test).