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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Neuropharmacology. 2010 Dec 25;60(6):842–851. doi: 10.1016/j.neuropharm.2010.12.024

Figure 3.

Figure 3

Effect of ghrelin on phalloindin-binding. Photomicrographs show phalloidin-based fluorescence representing polymerized F-actin in dendrites and spines in control, ghrelin, and D-Lys-3-GHSR-6. The graph depicts phalloidin-binding in response to ghrelin-application for 1 hour and 23 hours. The slices were fixed at the end of ghrelin-application. Some slices were kept alive before fixation for 22 hours in the absence of ghrelin after 1 hour of ghrelin-incubation (labeled as 1h+22h; see text for details). The calibration in Cont (10 µm) is shared by all three photomicrographs. Cont: Control, Ghr: Ghrelin, DLys3: D-Lys3-GHSR-6. Asterisks indicate p<0.01.