Figure 4.
Effects of endocannabinoids on ghrelin-induced pCREB expression. Photomicrographs show pCREB immunoreactivity in the control (A), 200 nM ghrelin (B), 200 nM ghrelin and 20 nM R(+)-methanandamide (C), 200 nM ghrelin, 20 nM R(+)-methanandamide and 5µM AM251 (D), 200 nM ghrelin, 20 nM R(+)-methanandamide and 5 µM capsazepine (E), 200 nM ghrelin and 5 µM AM251 (F), 200 nM ghrelin and 5 µM capsazepine (G), 200 nM ghrelin and 10 µM 2-AG(H), 200 nM ghrelin, 10 µM 2-AG and 5 µM AM251 (I). The graph in J summarizes the results. The calibration in I: 30 µm.