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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Free Radic Biol Med. 2011 Jan 14;50(8):945–952. doi: 10.1016/j.freeradbiomed.2011.01.010

Fig. 2. Hypoxia significantly increases ROS production in isolated complex III from PASMCs.

Fig. 2

(A) Effect of hypoxia on ROS formation (H2DCF fluorescence) in isolated complex III. (B) Representative Western blots show the presence of the complex III subunit Core 1 protein, but not the complex IV subunit COX IV protein in isolated complex III samples. (C) Effect of hypoxia on isolated complex III activity, gauged by assessing the absorbance of cytochrome c reduction at 550 nm.