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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Free Radic Biol Med. 2011 Jan 14;50(8):945–952. doi: 10.1016/j.freeradbiomed.2011.01.010

Fig. 3. Rieske iron-sulfur protein silencing blocks the hypoxic ROS production in isolated complex III from PASMCs.

Fig. 3

Fig. 3

(A) Effect of siRNA-mediated Rieske iron-sulfur protein silencing on its protein expression in PASMCs. Representative Western blots illustrate Rieske iron-sulfur protein and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression in PASMCs untransfected (control) and transfected with Rieske iron-sulfur protein siRNAs or scrambled siRNAs. Bar graph summarizes the effect of Rieske iron-sulfur protein siRNAs on Rieske iron-sulfur protein expression levels. *P<0.05 compared with untransfected cells (control). (B) Effect of Rieske iron-sulfur protein silencing on the acute hypoxic increase in the activity of isolated complex III. *P<0.05 compared with normoxic, untransfected samples; #P<0.05 compared with hypoxic, untransfected samples. (C) Effect of Rieske iron-sulfur protein silencing on the hypoxic increase in ROS formation in isolated complex III. (D) Effect of acute hypoxia on Rieske iron-sulfur protein expression in PASMCs.