Fig. 1.

a Schematic drawing of FRT mylz2-EGFP construct for assaying FLPe-mediated site-specific recombination. Black triangles represent FRT sites. The Tol2Kit (Kwan et al. 2007), which utilizes Gateway cloning (Invitrogen), was used to create this construct. Tol2 sites at either end of the construct facilitated zebrafish transgenesis. b Founder zebrafish embryos microinjected with the construct, FRT mylz2-EGFP, displayed mosaicism by 48 hpf. c Hemizygous F₁ embryos derived from mosaic founder fish that genotyped positive for the transgene in their sperm displayed even distribution of EGFP expression throughout