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Segregation ratios observed in the offspring derived from outcrossing confirmed FRT mylz2-EGFP transgenic fish that had been microinjected with FLPe-capped RNA at the single cell stage
| Recombinase microinjected | Parental phenotype | Offspring phenotype | Total | χ2 | P | |
|---|---|---|---|---|---|---|
| EGFP+ | EGFP− | |||||
| FLPe | Mosaic muscle-specific fluorescence | 34 (4.04%) | 806 (95.96%) | 842 | 711.5 | <0.001 |
| FLPe | No fluorescence | 0 (0%) | 339 (100%) | 339 | 339 | <0.001 |
