Figure 6.

PB-I-Surface Localization of GFP-PDIL2;3 Depends on crP10.

(A) The RNAi-mediated knockdown of crP10 in seeds (crP10-KD) was confirmed by immunoblot analysis with anti-crP10 antibody. The molecular mass of GFP-PDIL2;3 (predicted molecular mass = 76 kD) was confirmed by SDS-PAGE, followed by immunoblot analysis with anti-GFP antibody.

(B) Confocal fluorescence images of the crP10-KD endosperm (10 DAF) expressing spGFP-PDIL2;3. PB-I (arrowheads) and PB-II (asterisks) were visualized by Rhodamine staining. The fluorescence signals of GFP-PDIL2;3 (left panel) and Rhodamine (middle panel) were converted to green and red, respectively, and merged (right panel). Bars = 5 μm.