Figure 5.

ATX1 and TBP Interact in Vivo, and TBP Is Localized to Promoters.

(A) Nuclear extracts were immunoprecipitated with nonimmune IgG (IgG IP), ATX1 (ATX IP), or TBP antibodies (TBP IP). Immunoblots of the proteins immunoprecipitated by ATX1 antibody were analyzed with antibody to TBP (Anti TBP), while those immunoprecipitated by TBP antibody were analyzed with antibody to ATX1 (Anti ATX1). Representative immunoblots from three independent experiments are shown.

(B) A diagram of the gene regions analyzed by ChIP-PCR with TBP antibody or nonimmune IgG (see Supplemental Table 2 online for primer sequences).

(C) TBP or nonimmune IgG profiles of WRKY70 and LTP. The amount of DNA detected by ChIP-PCR, as a percentage of input DNA (y axis), is shown for each region. Experiments were repeated twice, and the bars represent the mean + se of a representative experiment, n = 3 replicates.

[See online article for color version of this figure.]