Figure 7.

Inhibitors of CDK7/CDK9-Like Kinases Diminish ATX1 Binding within Genes but Do Not Lower ATX1 or TBP Levels at Promoters.

The effects of genotype (wild type (WT) or atx1) or 6 h of treatment with mock or CDK7/CDK9 inhibitors (mock, Flap, or Selic) were analyzed by ChIP-PCR for changes in TBP or ATX1 occupancy on selected gene regions in leaves. The color key for the genotype or treatment is at the bottom of the figure. The gene name is shown above each panel, and the antibody or nonimmune IgG serum (IgG) used for ChIP-PCR is designated below the lanes.

(A) The effects of mock, Flap, or Selic treatments on ATX1 profiles on the WRKY70, NCED3, LTP, and ACT7 genes. The numbers on the x axis show the gene regions analyzed and correspond to the regions diagrammed in Figure 1A. Region 1 corresponds to the promoter region of each gene.

(B) The occupancy of TBP and ATX1 at the promoter region (region 1 in Figure 1A and/or in Supplemental Table 2 online) of three ATX1-regulated genes (WRKY70, NCED3, and LTP) and three genes not regulated by ATX1 (ACT7, TUB6, and ACT12). Experiments (A) were performed twice, and experiments in (B) were performed three times for WRKY70, NCED3, and LTP and twice for ACT7, TUB6, and ACT12. The bars represent the mean + se of a representative experiment, n = 3 replicates.