Figure 1.

Phenotypic Analysis of skb1-1 Mutant Plants Exposed to Salt Stress.

(A) and (B) Comparison of the survival rate of the wild type (Col-0) and the skb1-1 mutant grown on MS medium containing the indicated concentration of NaCl. Photographs were taken and the survival rate was determined 30 d after seedling transfer to the treatment medium. Data represent means ± (se of three independent experiments (n > 50).

(C) and (D) Comparison of root growth of wild type (Col-0), skb1-1, and 35S:SKB1 skb1-1 plants grown on MS medium with 100 mM NaCl. Four-day-old seedlings were transferred from MS medium to MS medium with 0 or 100 mM NaCl. Root growth was measured relative to controls 10 d after seedling transfer onto the treatment medium. More than 24 roots were measured for each data point. Data represent means ± se of three independent experiments.

(E) and (F) Comparison of the root growth of the wild type (Col-0) and skb1-1 mutant grown on MS medium with 100 mM KCl, 100 mM KNO₃, or 10 mM LiCl. Primary root length was measured and root growth relative to controls was analyzed 10 d after seedling transfer to the treatment medium. More than 24 roots were measured for each data point. Data represent means ± se of three independent experiments.

(G) Relative electrolyte leakage of leaves from wild-type (Col-0), skb1-1, and 35S:SKB1 Col-0 plants after exposure to 100 mM NaCl. Data represent means ± se of three independent experiments.