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. 2011 Jan 21;23(1):396–411. doi: 10.1105/tpc.110.081356

Figure 3.

Figure 3.

Loss of SKB1 Leads to Hypersensitivity to ABA.

(A) and (B) Seed germination of the wild type (Col-0), skb1-1, 35S:SKB1 skb1-1, and 35S:SKB1 Col-0. Seeds harvested on the same day were planted on MS medium containing 0, 0.2, 0.3, 0.5, or 1 μM ABA. Plates were transferred to a growth chamber after stratification at 4°C for 3 d. Photographs were taken, and germination rate was determined 3 d after the transfer. Data represent means ± se of three independent experiments (n = 96).

(C) and (D) Cotyledon greening of the wild type (Col-0), skb1-1, 35S:SKB1 skb1-1, and 35S:SKB1/Col-0. Photographs were taken and cotyledon greening rate was determined 6 d after transfer to the growth chamber. Data represent means ± se of three independent experiments (n = 96).

(E) Real-time quantitative RT-PCR analysis of the expression of salt stress and ABA-responsive genes in the wild type (Col-0) and skb1-1 and normalized with 18S rRNA expression. Error bars indicate the relative sd of three independent experiments. Template RNA isolated from 11-d-old seedlings growing on MS agar medium was treated with 200 mM NaCl for 6 h or 100 μM ABA for 3 h.