Figure 7.

LSM4 Structure and Pleiotropic Phenotypes of the lsm4 Mutant.

(A) LSM4 structure and the T-DNA insertion site of the lsm4 mutant (Salk_063398). White boxes represent exons or 5′ or 3′ untranslated regins, and black lines represent introns.

(B) RT-PCR analysis of LSM4 expression in 7-d-old wild-type (Col-0) and lsm4 seedlings. TUBULIN was used as a loading control.

(C) The phenotypes of 4-week-old wild-type (Col-0) and lsm4 mutant seedlings.

(D) The sensitivity of LSM4+/− F2 seedlings to ABA and salt stress. Photographs were taken after 8 d of culture at 23°C. Red arrows show nongreening seedlings or ungerminated seeds.

(E) Quantitative analysis of wild-type (Col-0) and LSM4+/− F2 greening cotyledon rate in MS medium containing 0.5 μM ABA and of germination rate in MS medium containing 100 mM NaCl, as shown in (D), n = 120.

(F) and (G) The pre-mRNA splicing pattern of genes, whose pre-mRNA splicing was affected by SKB1 mutation, in the lsm4 mutant.