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Detection of the Wild Type and Mutants of PSTVd Loop 6 in Petioles of Inoculated Leaves by RT-PCR.
The wild-type base pair is indicated by green color, mutant pairs capable of systemic infection by blue, and those incapable of systemic infection by red. M indicates mock inoculation. Asterisks indicate mutants that reverted to the wild type during infection. RT-PCR amplified actin RNA serves as loading control.
