Figure 10.

Destabilization of MCA1 by the Cytochrome f C Terminus Does Not Require the Presence of TCA1.

Accumulation of MCA1-HA, in an otherwise wild-type background (strain mH), in strains where petA coding sequence is expressed under the control of the atpA 5′UTR (strain mH {5′dAf}) and additionally carries a mutation preventing the CES process (strain mH {5′dAf₃₀₇S}). A dilution series is shown for strain mH {5′dAf₃₀₇S}. The same chloroplast genotypes were also compared in the mHt nuclear background (i.e., in the absence of TCA1). Accumulations of cytochrome f (Cyt. f) and subunit β of the mitochondrial ATP synthase (F1β, loading control) are also shown in the various strains.