Figure 5.

The Accumulation of MCA1 or TCA1 Depends on the Presence of Their Partners from the petA Gene Expression System.

(A) and (B) Immunoblots showing accumulation of TCA1-Fl in an otherwise wild-type genetic context (strain tF), in progeny expressing TCA1-Fl of one representative tetratype tetrad recovered after crossing mt and tF strains (A), or in the absence of petA mRNA (strain tF {ΔpetA}; [B]). The mtF progeny lacking MCA1 also lacks cytochrome f.

(C) Accumulation of MCA1-HA in strains with an otherwise wild-type genetic context (mH), lacking petA (mH {ΔpetA}), both petA and TCA1 (mHt {ΔpetA}), and in the progeny expressing MCA1-HA of one representative tetratype tetrad from the cross mt × mH. The mHt progeny that inherited the mutant tca1 allele from the mt parent lacks cytochrome f. A dilution series of the mH {ΔpetA} sample is shown for the ease of quantification.

For both panels, expression of cytochrome f (cyt. f) and of the β-subunit of the mitochondrial ATP synthase complex (F1β; loading control) is also shown. The wild-type strain (WT) that does not express MCA1-HA or TCA1-Fl is shown as a control.