Figure 7.

Disruption of the CES Repressor Domain Stabilizes MCA1.

(A) Schematic representation of the mutations introduced in the stromal-exposed C-terminal tail of cytochrome f in strain mH. Substitutions of the residues shaded in black abolish the cytochrome f CES process, whereas mutations of those shaded in gray attenuate this regulatory process (Choquet et al., 2003).

(B) Accumulation of MCA1-HA in strains with an otherwise wild-type background (strain mH) or carrying the mutations depicted in (A). A dilution series of the strain mH {f_sol} is shown for the ease of quantification. Note that strain mH {fΔK} was slightly overloaded in this gel. Cyt., cytochrome.