Accumulation and Half-Life of MCA1 Are Governed by the Accumulation of the CES Repressor Motif.
(A) Accumulation of MCA1-HA and cytochrome f (Cyt. f) in a double mutant mH, {ccsA-B6}, and in the parental strains mH and {ccsA-B6}.
(B) Accumulation of MCA1-HA and cytochrome f in strains mH and mH {ΔpetD}.
In both panels, the accumulation of the β-subunit of the mitochondrial ATP synthase complex (F1β) provides a loading control.
(C) Half-life of MCA1-HA assessed in strains mH and mH {ΔpetD} by immunochase experiments in the presence of cycloheximide (C.) or both cycloheximide and lincomycin (C.+L.).