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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Protein Expr Purif. 2011 Jan 22;77(1):34–45. doi: 10.1016/j.pep.2011.01.007

Figure 6.

Figure 6

Expression analysis of flavivirus NS1 protein varying the secretion signal. Various NS1-MBP fusions were purified from the media or cell pellets of recombinant baculovirus-infected insect cell cultures and analyzed with the Labchip 90. Protein from the media of infected High-five (A) or Sf9 (B) cell cultures was buffered exchanged by spin-filtration into phosphate-buffered saline and then subjected to nickel chelate chromatography. Cell pellets from High-five (C) or Sf9 (D) cultures were resuspended in lysis buffer, the soluble fraction was separated from the insoluble fraction and then subjected to nickel chelate chromatography. Samples from the eluates were then run on the Labchip 90. Molecular weight markers are in the M lanes. Signal peptide sequences, viral origins and construct amino acid compositions are indicated. The WNV constructs have N- and C-terminal truncations and the JEV constructs have a C-terminal truncation as described in the text. The arrows indicate protein bands consistent with the expected molecular weight of mature, processed or unprocessed NS1 fusion protein.