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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Protein Expr Purif. 2011 Jan 22;77(1):34–45. doi: 10.1016/j.pep.2011.01.007

Figure 8.

Figure 8

Characterization of the aggregation state of purified JEV MBP-NS1. A. Size exclusion chromatography profile for MBP-NS1 (JEV) fusion protein purified from conditioned media. The column void volume is indicated. Molecular weights of peaks were assigned by fitting elution volumes to a standard curve. B. SDS-PAGE analysis. 30 μL aliquots of fractions from Peak 1 (180 kDa ), lane 1, and Peak 2 (103 kDa), lanes 2 and 3, were analyzed on a 4–20 % gradient gel that was stained with Coomassie blue. The MBP-NS1 fusion protein is indicated with an arrow.