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. Author manuscript; available in PMC: 2011 Mar 9.
Published in final edited form as: Nature. 2010 Sep 9;467(7312):214–217. doi: 10.1038/nature09337

Figure 3.

Figure 3

Dendritic cell activation by HIV-1 requires IRF-3. (a) Tubulin, Histone H3, IRF3 and Phosphor-Ser396-IRF3 expression in cytoplasmic and nuclear fractions of MDDC infected with SIVVLP(G) and dilutions of HIVGFP(G) in the presence or the absence of CsA. Cells were harvested at 8 hours after infection or after control treatment with poly(I:C). (b) GFP, RFP and CD86 expression in MDDC initially transduced with GFP-encoding control shRNA vector or a shRNA vector targeting IRF3 and subsequently challenged with HDVIRESRFP(G) or treated with poly(I:C) or Curdlan. Right panel: cells are gated on GFP+ transduced populations.