Table 2.

Enzymatic hydrolysis of 3-methoxy-4-hydroxyphenylglycol conjugate isolated from rat brain^*

Enzyme treatment	Ethyl acetate extractable radioactivity(cpm)
None (control)	6200
β-Glucuronidase and sulfatase(glusulase)	54,200
plus p-nitrocatechol sulfate	7300
plus p-nitrocatechol phosphate	51,000
β-Glucuronidase	5700
Acid phosphatase	6200

^*The radioactive conjugate found in rat brain after the intracisternal injection of normetanephrine-H³ was isolated (see text). This conjugate was incubated under various conditions (control, enzyme, and enzyme plus inhibitors). Hydrolysis was estimated by measuring the radioactivity extracted from the incubate into ethyl acetate at pH 7. Each value represents the mean of 4 determinations. p-Nitrocatechols were used at a concentration of 1 × 10⁻²M.