Table 4.

Parameters of [³⁵S]GTPγS binding to G_i/o, G_s/olf and G_q/11 subtypes of G-proteins

	Gi/o		Gs/olf		Gq/11
	pEC50	EMAX	pEC50	EMAX	pEC50	EMAX
carbachol	5.11 ± 0.06	2.89 ± 0.06	4.25 ± 0.06	1.82 ± 0.05	4.37 ± 0.02	1.61 ± 0.02
furmethide	5.34 ± 0.10	2.95 ± 0.09	4.76 ± 0.03*	1.70 ± 0.02	4.72 ± 0.06*	1.20 ± 0.01*
oxotremorine	6.03 ± 0.05**	2.72 ± 0.05	5.13 ± 0.04**	1.53 ± 0.01**	n.c.	n.c.***
pilocarpine	5.95 ± 0.06**	1.52 ± 0.05***	5.05 ± 0.05**	1.10 ± 0.01***	4.95 ± 0.05**	1.08 ± 0.01***

Constants and Hill coefficients (nH) were obtained by fitting Equation 2 to data from individual experiments shown in Figure 1. Half effective molar concentration of agonists is expressed as negative logarithm (pEC₅₀) and maximal stimulation (E_MAX) as fold increase over basal binding. Data are means ± SEM of values from three individual experiments performed in quadruplicates.

^*P < 0.05, significantly different from carbachol;

^**P < 0.01, significantly different from carbachol and furmethide;

^***P < 0.001, significantly different from all other agonists by anova and Tukey's test.

GTPγS, guanosine-5′-γ−thiotriphosphate; n.c., no convergence.