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. 2011 Mar;162(5):1083–1095. doi: 10.1111/j.1476-5381.2010.01108.x

Figure 2.

Figure 2

Sema3A-induced growth cone collapse occurs only in sympathetic and parasympathetic neurones expressing Nrp-1. (A–D) Confocal images of growth cones from sympathetic (TH-positive: A, intact; B, collapsed) and parasympathetic (NOS-positive: C, intact; D, collapsed) pelvic ganglion neurones expressing Nrp-1. Merged images of TH and NOS with Nrp-1 immunostaining are shown in (B) and (D) respectively. Arrowhead in (D) shows an Nrp-1-negative collapsed growth cone in close proximity to an Nrp-1-positive collapsed growth cone. (E, F) Sema3A caused growth cone collapse in sympathetic (E) and parasympathetic (F) neurones with Nrp-1-immunoreactivity (Nrp-1-IR), but did not cause collapse in Nrp-1-negative neurones (n= 3). Tukey's test: ***P < 0.001 versus Sema3A-negative control. Scale bar = 10 µm in (A–D). NOS, nitric oxide synthase; Nrp-1, neuropilin-1; Sema3A, semaphorin 3A; TH, tyrosine hydroxylase.