Figure 3.

Effect of 1-(15-HPEPE)-lysoPC, in combination with 15-LOX inhibitor (PD146176), on zymosan A-induced plasma leakage in mice. 1-(15-HPEPE)-lysoPC (0–150 µg·kg⁻¹) and PD146176 (100 mg·kg⁻¹), dissolved in phosphate-buffered saline (PBS), were administered i.v. and i.p., respectively, to mice 30 min prior to i.v. administration of Evans blue dye, followed by i.p. administration of zymosan A (100 mg·kg⁻¹). After 60 min, the peritoneum was lavaged with sterile cold PBS, and the plasma leakage was determined as described in Methods. Values are means ± SEM (n= 7–10). *P < 0.05; **P < 0.01, versus zymosan A-treated group; ###P < 0.001 versus PBS-treated group (one-way anova followed by the Newman–Keuls-Student's test).