Figure 6.

Time-dependent effect of 15-HEPE-lysoPC on zymosan A-induced plasma leakage and leucocyte infiltration. (A) Mice was administered either 15-HEPE-lysoPC (50 µg·kg⁻¹) or phosphate-buffered saline (PBS) 30 min prior to i.v. administration of Evans blue dye, followed by zymosan A treatment. At indicated time points (0.5–24 h) after zymosan A challenge, lavage was collected and plasma leakage was determined as described in Figure 2. (B) Mice were administered i.p. either 15-HEPE-lysoPC (50 µg·kg⁻¹) or PBS 30 min prior to zymosan A treatment. At different time points after zymosan A challenge, lavage was collected and leucocyte infiltration was assessed by using light microscopy and a Neubauer chamber. Values are means ± SEM (n= 5). *P < 0.05, **P < 0.01, ***P < 0.01 versus PBS treated group (one-way anova followed by the Newman–Keuls-Student's test).